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KMID : 0665220100230030306
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Korean Journal of Food and Nutrition
2010 Volume.23 No. 3 p.306 ~ p.310
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Protective Effect of Co-treatment of Lutein and Fucoidan Against AAPH-Induced Damage in THP-1 Cells
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Lee Kyeong-Ho
Yoon Wan-Ho
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Abstract
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This study was designed to investigate the protective effect of the combination of fucoidan and lutein against AAPH-induced oxidative stress in THP-1 cells. The combination of fucoidan and lutein existed significant antioxidant effect on AAPHdamaged THP-1 cells by using lipid peroxidation and cellular antioxidant capacity assay. Fucoidan(1 ㎍/㎖) and lutein(10 ㎍/ ㎖) did not affect at all the viability of THP-1 cells, but protected the AAPH-damage of THP-1 cells at the same concentration. The viability of THP-1 cells was 0% with 1 mM AAPH alone, the protective effect of fucoidan(1 ㎍/㎖) and lutein(10 ㎍/㎖) was 37% and 36%, respectively. The combination of fucoidan(1 ㎍/㎖) and lutein(10 ㎍/㎖) exhibited significant inhibitory effect of lipid peroxidation using TBARS assay and cellular antioxidant capacity using DCFH-DA assay. In lipid peroxidation, the TBARS value of 1 mM AAPH alone was 0.8±0.03 nM MDA, its of the combination of fucoidan(1 ㎍/㎖) and lutein(10 ㎍/㎖) was 0.2±0.05 nM MDA. In cellular antioxidant capacity, the combination of fucoidan(1 ㎍/㎖) and lutein(10 ㎍/㎖) exhibited significant cellular antioxidant capacity of 76%, whereas quercetin(10 μM) as positive control exhibited the cellular antioxidant capacity of 32%. These results indicate that the cotreatment of fucoidan and lutein protects against AAPH-induced THP-1 cell damage by inhibiting lipid peroxidation, increasing cellular antioxidant capacity.
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KEYWORD
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lutein, fucoidan, antioxidant, THP-1 cell line
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